Rabbit polyclonal antibody to HES6Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentation.
Immunogen
The antiserum was produced against synthesized peptide derived from human HES6.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
C-HAIRY1 CHAIRY1 Hairy and enhancer of split6 Hairy and enhancer of split 6 HES6 C HAIRY1
Target
HES6
Molecular Weight(MW)
24 kDa (note)
Tissue Specificity
HES6 Antibody detects endogenous levels of total HES6 protein.
Application
Application
Western blot analysis of extracts from HeLa cells, using HES6 Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.