HES6 belongs to a subfamily of basic helix-loop-helix transcription factors that includes Drosophila Hairy and Enhancer of split genes. Like other members of the family, HES6 features the WRPW motif which is consisted just of four amino acids at its C-terminus. It is expressed by both undifferentiated and differentiated cells and is an inhibitor of Hes1. HES6 supports Mash1 activity and promotes cell differentiation.1) Kang, S.A., (2005) Biochem. Biophys. Res. Commun. 332 (1), 33-36.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HES6.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
HES6 antibody can be used for detection of HES6 by ELISA at 1:62500. HES6 antibody can be used for detection of HES6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Transfected 293T at 1.25 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
HES6 antibody can be used for detection of HES6 by ELISA at 1:62500. HES6 antibody can be used for detection of HES6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HES6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt