Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HET/SAF-B [6F7] antibody at 1:200 dilution.Application
Detection of human HET SAF-B protein in HeLa whole cell lysate using GeneTex monoclonal antibodyApplication
HET/SAF-B antibody [6F7] detects HET/SAF-B protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 5% SDS-PAGE HET/SAF-B antibody [6F7] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | HET/SAF-B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human HET / SAF - BHsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues. |
| Immunogen | A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6F7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| For ICC/IF: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1 - 2 µg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp779C1727 SAFB SAFB1 HET 6294 HET/SAFB Q15424 HAP 602895 HET/SAF B |
|---|---|
| Target | HET/SAF-B |
| Molecular Weight(MW) | 102 kDa (note) |
| Light Chain Type | kappa |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HET/SAF-B [6F7] antibody at 1:200 dilution.
Application
Detection of human HET SAF-B protein in HeLa whole cell lysate using GeneTex monoclonal antibody
Application
HET/SAF-B antibody [6F7] detects HET/SAF-B protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 5% SDS-PAGE HET/SAF-B antibody [6F7] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | HeLa, MCF7 |
|---|---|
| Application Notes | For ICC/IF: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1 - 2 µg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
