Application
Immunofluorescent staining of HT29 cells using anti-HHex mouse monoclonal antibody .Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HHex monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HHex (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HHex.| Product Name | Hex antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3E6] to HexRecognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels | acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. |
| Immunogen | Recombinant fragment expressed in E.coli corresponding to amino acids 100-270 of human Hex |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3E6 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HEX PRH PRHX HOX11LPEN HOX11L-PEN HMPH HHEX HOX11L PEN |
|---|---|
| Target | Hex |
| Tissue Specificity | is specific for amino acids 100-270 of human Hex |
Application
Immunofluorescent staining of HT29 cells using anti-HHex mouse monoclonal antibody .
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HHex monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HHex (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HHex.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
