Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using HXK I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HXK I antibody at 1:250 dilution.Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 C:HeLa S3 D:Hep G2 7.5% SDS PAGE diluted at 1:3000| Product Name | Hexokinase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HXK I (hexokinase 1)Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HXK I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, BovineMouse (94%), Rat (92%), Zebrafish (84%), Xenopus laevis (86%), Pig (93%), Chicken (88%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HK1-ta antibody HK1-tb antibody HK1-tc antibody HKI antibody HXK1 antibody HK1 antibody glycolytic enzyme antibody hexokinase type I antibody HK I antibody brain form hexokinase antibody hexokinase-1 antibody hexokinase 1 antibody |
|---|---|
| Molecular Weight(MW) | 102 kDa (note) (9) |
| Cellular Localization | Mitochondrion outer membrane |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using HXK I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HXK I antibody at 1:250 dilution.
Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 C:HeLa S3 D:Hep G2 7.5% SDS PAGE diluted at 1:3000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
