Application
Flow cytometric analysis of K562 cells using HK1 mouse mAb ( green) and negative control (purple)Application
Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb ( green). Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidinApplication
Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 mouse mAb with DAB staining.Application
Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.| Product Name | Hexokinase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3A10] to HK1Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009] |
| Immunogen | The immunogen corresponding to human HK1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3A10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | HK1-TC HKI HK1-TA HK1-TB HXK1 Hexokinase 1 HK1 antibody |
|---|---|
| Target | HK1 |
| Molecular Weight(MW) | 120 kDa (note) |
Application
Flow cytometric analysis of K562 cells using HK1 mouse mAb ( green) and negative control (purple)
Application
Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb ( green). Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin
Application
Immunohistochemical analysis of paraffin-embedded kidney tissues using HK1 mouse mAb with DAB staining.
Application
Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
