Application
WB analysis of (A) recombinant protein (20ng or 100ng) of Hexokinase four isoform and (B) the indicated lysates (20ug) using Hexokinase antibody at a dilution of 1:1,000.Application
ICC/IF analysis of HeLa cells using Hexokinase antibody at a dilution of 1:1,000 (green) and Hoechst 33342 (blue).| Product Name | Hexokinase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4D7] to HexokinaseHexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009] |
| Immunogen | Recombinant human Hexokinase1 (1-917aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 4D7 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein. The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence assay to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HK1-tb HKI HK1-tc hexokinase 1 HK1-ta HXK1 HK1 |
|---|---|
| Target | Hexokinase |
| Light Chain Type | kappa |
Application
WB analysis of (A) recombinant protein (20ng or 100ng) of Hexokinase four isoform and (B) the indicated lysates (20ug) using Hexokinase antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of HeLa cells using Hexokinase antibody at a dilution of 1:1,000 (green) and Hoechst 33342 (blue).| Application Notes | The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein. The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence assay to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1,000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
