Application
(A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase 3 (1:1000). (B): Cell lysates (30?g) were resolved by SDS-PAGE, transferred to NC membrane and| Product Name | Hexokinase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4F5] to Hexokinase 3Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase 3 may have anabolic functions, providing H6P for glycogen or lipid synthesis. |
| Immunogen | Recombinant human Hexokinase 3 (1-923aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4F5 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HK3 HK III HXK3. Hexokinase 3 EC2.7.1.1 HKIII Hexokinase3 EC 2.7.1.1 Hexokinase type III Hexokinase-3 |
|---|---|
| Target | Hexokinase 3 |
| Light Chain Type | kappa |
Application
(A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase 3 (1:1000). (B): Cell lysates (30?g) were resolved by SDS-PAGE, transferred to NC membrane and| Application Notes | Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
