Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Hexokinase II antibody detects HK2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: HK2 protein stained by Hexokinase II antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 µg of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (20 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Hexokinase II antibody dilution: 1:500.Application
Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Hexokinase II antibody diluted at 1:500.Application
Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Hexokinase II antibody dilution: 1:500.Application
Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Hexokinase II antibody diluted at 1:500.Application
Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat stomach by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Hexokinase II antibody diluted at 1:500.| Product Name | Hexokinase II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Hexokinase II (hexokinase 2)Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Hexokinase II. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Pig, ChickenZebrafish (86%), Pig (96%), Chicken (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686M1669 antibody HKII antibody HXK2 antibody HK2 antibody HK II antibody hexokinase type II antibody muscle form hexokinase antibody hexokinase-2 muscle antibody hexokinase-2 antibody hexokinase 2 antibody |
|---|---|
| Molecular Weight(MW) | 102 kDa (note) |
| Cellular Localization | Mitochondrion outer membrane (By similarity) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Hexokinase II antibody detects HK2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: HK2 protein stained by Hexokinase II antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 µg of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (20 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Hexokinase II antibody dilution: 1:500.
Application
Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Hexokinase II antibody diluted at 1:500.
Application
Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Hexokinase II antibody dilution: 1:500.
Application
Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Hexokinase II antibody diluted at 1:500.
Application
Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat stomach by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Hexokinase II antibody diluted at 1:500.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
