Application
WB analysis of PC12, HeLa and Jurkat lysates using Hexokinase-2 antibody at a dilution of 1:1,000.Application
FACS analysis of K562 cells using Hexokinase-2 antibody at a dilution of 1:100 (red) or an isotype control (blue).| Product Name | Hexokinase II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1E8-H3-F11] to Hexokinase IIHexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009] |
| Immunogen | Purified recombinant human Hexokinase II ( N-terminus) protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E8-H3-F11 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| FACS:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HK2 HKII hexokinase 2 HXK2 |
|---|---|
| Target | Hexokinase II |
| Molecular Weight(MW) | 102kDa (note) |
| Cellular Localization | Mitochondrion outer membrane |
Application
WB analysis of PC12, HeLa and Jurkat lysates using Hexokinase-2 antibody at a dilution of 1:1,000.
Application
FACS analysis of K562 cells using Hexokinase-2 antibody at a dilution of 1:100 (red) or an isotype control (blue).| Application Notes | FACS:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
