Application
Anti-HK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HK2Application
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody .Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-HK2mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HK2mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells transfected with either pCMV6-ENTRY HK2Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-HK2mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.| Product Name | Hexokinase II antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4C5] to HK2Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4C5 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanDog |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HK2 HKII HXK2 DKFZp686M1669 |
|---|---|
| Target | HK2 |
| Tissue Specificity | is specific for human HK2 (NP_000180). |
Application
Anti-HK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HK2
Application
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody .
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-HK2mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HK2mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells transfected with either pCMV6-ENTRY HK2
Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-HK2mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
