Application
A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC| Product Name | Hexokinse 1, 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1A7 ] to Hexokinse 1, 2Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. |
| Immunogen | Recombinant human Hexokinase 2 (1-917aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1A7 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000 ~ 3,000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Hexokinase type II Hexokinase2 Hexokinase HK2 HK1-tc Hexokinase 2 Hexokinase PI tumor isozyme HK1tc HexokinaseA mHk1-s. HKI BB404130 Hexokinase-1 Hexokinase type I HK1ta HK1 dea Hexokinase1 mHk1s. HK1 tc mHk1 s. Hexokinase 1 |
|---|---|
| Target | Hexokinse 1, 2 |
| Light Chain Type | kappa |
Application
A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC| Application Notes | Western Blot: Use at 1:1,000 ~ 3,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
