Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with HGF (alpha subunit) antibody diluted at 1:500 and competitor's antibody (SC-7949) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HGF (alpha subunit) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HGF (alpha subunit)Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human HGF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cat, Rabbit, BovineMouse (84%), Rat (85%), Dog (91%), Cat (91%), Rabbit (89%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DFNB39 antibody F TCF antibody HGF antibody HGFB antibody HPTA antibody SF antibody hepatocyte growth factor antibody |
|---|---|
| Target | HGF (alpha subunit) |
| Molecular Weight(MW) | 83 kDa (note) (5) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with HGF (alpha subunit) antibody diluted at 1:500 and competitor's antibody (SC-7949) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Huh7 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
