Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using HGS antibody (green) at 1:200 dilution. Alpha-tubulin filaments were labeled with (red) at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HGS antibody at 1:500 dilution.Application
Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HGS antibody [C2C3], C-term detects HGS protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE HGS antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HGS antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HGS (hepatocyte growth factor-regulated tyrosine kinase substrate)The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HGS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Rhesus Monkey, BovineDog (93%), Rhesus Monkey (98%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ11421 antibody FLJ39878 antibody HRS antibody HGS antibody protein pp110 antibody human growth factor-regulated tyrosine kinase substrate antibody hepatocyte growth factor-regulated tyrosine kinase substrate antibody |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) |
| Cellular Localization | Cytoplasm , Early endosome membrane; Peripheral membrane protein; Cytoplasmic side , Endosome , multivesicular body membrane; Peripheral membrane protein |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using HGS antibody (green) at 1:200 dilution. Alpha-tubulin filaments were labeled with (red) at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HGS antibody at 1:500 dilution.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HGS antibody [C2C3], C-term detects HGS protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE HGS antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: HeLa 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | H1299 , HeLa , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
