Application
HHIP antibody [N3C2], Internal detects HHIP protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HHIP antibody [N3C2], Internal antibody detects HHIP protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde for 15 min. Green: HHIP protein stained by HHIP antibody [N3C2], Internal antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
HHIP antibody [N3C2], Internal detects HHIP protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HHIP antibody [N3C2], Internal dilution: 1:500.| Product Name | HHIP antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HHIP (hedgehog interacting protein)This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HHIP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseChicken |
| Predicted Reactivity | Rat, Xenopus laevis, Rhesus Monkey, BovineRat (98%), Xenopus laevis (83%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20992 antibody FLJ90230 antibody HIP antibody HHIP antibody hedgehog-interacting protein antibody hedgehog interacting protein antibody |
|---|---|
| Molecular Weight(MW) | 79 kDa (note) |
| Cellular Localization | Cell membrane; Peripheral membrane protein (By similarity) , Secreted (By similarity) , Isoform 2: Cytoplasm |
Application
HHIP antibody [N3C2], Internal detects HHIP protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HHIP antibody [N3C2], Internal antibody detects HHIP protein by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde for 15 min. Green: HHIP protein stained by HHIP antibody [N3C2], Internal antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
HHIP antibody [N3C2], Internal detects HHIP protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HHIP antibody [N3C2], Internal dilution: 1:500.| Positive Control | Mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
