Application
WB analysis of various cell lysates using hHR23b antibody [5H1-A10-A7] at a dilution of 1:1000.Application
ICC/IF analysis of HeLa cells using hHR23b antibody [5H1-A10-A7] at a dilution of 1:100.Application
IHC-P analysis of prostate cancer using hHR23b antibody [5H1-A10-A7] at a dilution of 1:100. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).| Product Name | hHR23b antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [5H1-A10-A7] to hHR23bThe protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011] |
| Immunogen | Purified recombinant human hHR23b protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 5H1-A10-A7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
|
For WB: Use at a dilution of 1:1000. : |
|
| Species Reactivity | HumanMouseMonkeyRatHamster |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RAD23B P58 HR23B HHR23B RAD23 homolog B (S. cerevisiae) |
|---|---|
| Target | hHR23b |
Application
WB analysis of various cell lysates using hHR23b antibody [5H1-A10-A7] at a dilution of 1:1000.
Application
ICC/IF analysis of HeLa cells using hHR23b antibody [5H1-A10-A7] at a dilution of 1:100.
Application
IHC-P analysis of prostate cancer using hHR23b antibody [5H1-A10-A7] at a dilution of 1:100. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).| Application Notes |
For WB: Use at a dilution of 1:1000. : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. |
