Application
Immunofluorescence analysis of methanol-fixed A431, using HIBCH antibody at 1:200 dilution.Application
HIBCH antibody detects HIBCH protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. HIBCH antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: Molt-4 10% SDS PAGE diluted at 1:1000| Product Name | HIBCH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIBCH (3-hydroxyisobutyryl-CoA hydrolase)Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.[supplied by OMIM] |
| Immunogen | Full length human HIBCH Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (81%), Bovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIBYLCOAH antibody HIBCH antibody HIBYL-CoA-H antibody HIB-CoA hydrolase antibody 3-hydroxyisobutyryl-CoA hydrolase mitochondrial antibody 3-hydroxyisobutyryl-Coenzyme A hydrolase antibody 3-hydroxyisobutyryl-CoA hydrolase antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunofluorescence analysis of methanol-fixed A431, using HIBCH antibody at 1:200 dilution.
Application
HIBCH antibody detects HIBCH protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. HIBCH antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: Molt-4 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , HepG2 , Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
