Application
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody, and then analyzed by flow cytometry.Application
Anti-HIBCH mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HIBCHApplication
Flow cytometric Analysis of Jurkat cells, using anti-HIBCH antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.| Product Name | HIBCH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1C7] to HIBCHHydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA. |
| Immunogen | Full length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1C7 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | HumanDog |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIBYL COA H HIBYLCOAH |
|---|---|
| Target | HIBCH |
| Tissue Specificity | is specific for human HIBCH (NP_055177). |
Application
HEK293T cells transfected with either RC209814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HIBCH antibody, and then analyzed by flow cytometry.
Application
Anti-HIBCH mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HIBCH
Application
Flow cytometric Analysis of Jurkat cells, using anti-HIBCH antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
