Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using HIC5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 polyclonal antibody. A: Non-transfected lysate. B: TGFB1I1 transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | HIC5 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIC5 (transforming growth factor beta 1 induced transcript 1)This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIC5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus MonkeyMouse (100%), Rat (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARA55 antibody HIC-5 antibody HIC5 antibody TSC-5 antibody TGFB1I1 antibody hydrogen peroxide-inducible clone 5 protein antibody androgen receptor coactivator 55 kDa protein antibody transforming growth factor beta-1-induced transcript 1 protein ant |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) |
| Cellular Localization | Cell junction , focal adhesion , Nucleus matrix , Cytoplasm , cytoskeleton |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using HIC5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Western Blot analysis of TGFB1I1 expression in transfected 293T cell line by TGFB1I1 polyclonal antibody. A: Non-transfected lysate. B: TGFB1I1 transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | Hic-5 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
