Application
Sample (30 ug of whole cell lysate) A: 293T B: HeLa 10% SDS PAGE diluted at 1:1000| Product Name | Hice1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [15A2-35] to Hice 1This antibody binds to Hice 1, or HAUS8, which is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 15A2-35 |
| Host Species | Mouse |
| Tested Applications | WB |
| Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC20533 NYSAR48 HICE1 HAUS8 NY-SAR-48 NY SAR 48 |
|---|---|
| Target | Hice 1 |
| Cellular Localization | During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, additionally associates with the spindle midzone and midbody, respectively. |
Application
Sample (30 ug of whole cell lysate) A: 293T B: HeLa 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , HeLa |
|---|---|
| Application Notes | Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short term use, store at 4°C. For extended use, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS |
