Application
Immunohistochemical staining of human globlastoma multiforme utilizingApplication
Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-HIF-1 alpha antibody [H1alpha67] .Application
MEF cells were grown on 15 cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs.Application
HIF-1 alpha Antibody [H1alpha67] - Detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.Application
Immunoprecipitation using HIF-1 alpha Antibody [H1alpha67] - Heavy and light chains are also detected.Application
Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.Application
Western Blot: HIF-1 alpha Antibody (H1alpha67) - Analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts| Product Name | HIF1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [H1alpha67] to Hypoxia Inducible Factor-1 alphaHypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers. |
| Immunogen | Amino acids 432-528 of human HIF-1alpha. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | H1alpha67 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSGSAICC/IFIHCIHC-FIHC-PIPWBChIP |
| For WB: Use at a dilution of 1:500.: |
|
| Species Reactivity | HumanMousePigRatYeastFerretPrimateRabbitSheepBovine |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | HIF1 alpha HIF-1alpha HIF1A MOP1 PASD8 HIF1-ALPHA 603348 HIF1ALPHA Q16665 3091 HIF 1alpha |
|---|---|
| Target | HIF1 alpha |
| Tissue Specificity | This antibody is specific for HIF-1 alpha. |
| Cellular Localization | HIF-1 is a nuclear protein. |
Application
Immunohistochemical staining of human globlastoma multiforme utilizing
Application
Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-HIF-1 alpha antibody [H1alpha67] .
Application
MEF cells were grown on 15 cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs.
Application
HIF-1 alpha Antibody [H1alpha67] - Detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.
Application
Immunoprecipitation using HIF-1 alpha Antibody [H1alpha67] - Heavy and light chains are also detected.
Application
Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
Application
Western Blot: HIF-1 alpha Antibody (H1alpha67) - Analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts| Positive Control | Nuclear extracts recommended |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline containing 0.02% sodium azide |
