Application
Western blot analysis of HIF1 beta in HeLa nuclear extract.Application
Immunohistochemical staining of human glioblastoma multi-forme using anti-HIF1 beta antibody [H1beta234].| Product Name | HIF1 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [H1beta234] to HIF1 beta / ARNTHypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF1 is a heterodimer composed of HIF1 alpha and HIF1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF1 alpha increases in the cell. In contrast, HIF1 beta remains stable under either condition. HIF1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. |
| Immunogen | Fusion protein containing amino acids 496-789 of human HIF1 beta |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | H1beta234 |
| Host Species | Mouse |
| Tested Applications | GSAICC/IFIHC-PIPWBChIP |
| For WB: Use at a dilution of 1:500 (ECL). Used in Western blot this antibody recognizes a band at approximately 92kD representing HIF-1Beta. For IHC-P: Use at a dilution of 1:100. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRatFerretSheep |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | ARNT 126110 HIF1 beta HIF-1beta HIF1beta P27540 TANGO HIF1B 405 HIF 1beta |
|---|---|
| Target | HIF1 beta |
| Tissue Specificity | This antibody is specific to HIF1 beta /ARNT |
| Light Chain Type | kappa |
Application
Western blot analysis of HIF1 beta in HeLa nuclear extract.
Application
Immunohistochemical staining of human glioblastoma multi-forme using anti-HIF1 beta antibody [H1beta234].| Application Notes | For WB: Use at a dilution of 1:500 (ECL). Used in Western blot this antibody recognizes a band at approximately 92kD representing HIF-1Beta. For IHC-P: Use at a dilution of 1:100. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution.Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.05% sodium azide |
