Application
Hif1ab antibody detects Hif1ab protein by western blot analysis. Un-treated (-) and treated (+, UVC treatment for ) zebrafish embryos whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hif1ab antibody at a dilution of 1:1000.| Product Name | Hif1ab antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Hif1ab (hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) b) |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of zebrafish Hif1ab. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hypoxia-inducible factor 1 alpha subunit (basic helix-loop-helix transcription factor) b hif1a MGC55405 zgc:55405 hif1ab wu:fb63a10 fb63a10 |
|---|---|
| Target | Hif1ab |
Application
Hif1ab antibody detects Hif1ab protein by western blot analysis. Un-treated (-) and treated (+, UVC treatment for ) zebrafish embryos whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hif1ab antibody at a dilution of 1:1000.| Positive Control | zebrafish embryos (untreated) , zebrafish embryos (treated UV irradiation) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
