Application
HIF1AN antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1AN protein stained by HIF1AN antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: HCT116 B: MCF-7 10% SDS PAGE diluted at 1:1000Application
HIF1AN antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1AN protein stained by HIF1AN antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
HIF1AN antibody detects HIF1AN protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. HIF1AN antibody dilution: 1:500.| Product Name | HIF1AN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIF1AN (hypoxia inducible factor 1, alpha subunit inhibitor)Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HIF1AN. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (98%), Rat (98%), Zebrafish (86%), Xenopus laevis (90%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp762F1811 antibody FIH1 antibody FLJ20615 antibody FLJ22027 antibody HIF1AN antibody FIH-1 antibody factor inhibiting HIF1 antibody peptide-aspartate beta-dioxygenase antibody factor inhibiting HIF-1 antibody hypoxia-inducible factor 1-alpha i |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
HIF1AN antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1AN protein stained by HIF1AN antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: HCT116 B: MCF-7 10% SDS PAGE diluted at 1:1000
Application
HIF1AN antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1AN protein stained by HIF1AN antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
HIF1AN antibody detects HIF1AN protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. HIF1AN antibody dilution: 1:500.| Positive Control | HCT116 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
