Application
HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: HIF2 alpha protein stained by HIF2 alpha antibody diluted at 1:300. Red: alpha Tubulin protein stained by alpha Tubulin antibody diluted at 1:1000. Scale bar = 10 μm.| Product Name | HIF2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT1689] to HIF2 alpha (endothelial PAS domain protein 1)This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT1689 |
| Host Species | Mouse |
| Tested Applications | ICC/IF |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, PigMouse (80%), Pig (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EPAS1 ECYT4 HIF2A HLF bHLHe73 MOP2 endothelial PAS domain protein 1 PASD2 |
|---|---|
| Target | HIF2 alpha |
| Molecular Weight(MW) | 96 kDa (note) |
| Cellular Localization | Nucleus |
Application
HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: HIF2 alpha protein stained by HIF2 alpha antibody diluted at 1:300. Red: alpha Tubulin protein stained by alpha Tubulin antibody diluted at 1:1000. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
