Application
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract (1% O2 treatment for 24 hr) 7.5% SDS-PAGE HIF2 alpha antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HIF2 alpha antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIF2 alpha (endothelial PAS domain protein 1)This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanZebra Fish |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, BovineMouse (97%), Rat (95%), Xenopus laevis (86%), Pig (99%), Chicken (94%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ECYT4 antibody HIF2A antibody HLF antibody MOP2 antibody PASD2 antibody bHLHe73 antibody EPAS1 antibody hypoxia-inducible factor 2-alpha antibody HIF-2-alpha antibody hypoxia-inducible factor 2 alpha antibody member of PAS protein 2 antibody EPA |
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| Molecular Weight(MW) | 96 kDa (note) |
| Cellular Localization | Nucleus |
Application
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract (1% O2 treatment for 24 hr) 7.5% SDS-PAGE HIF2 alpha antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 (1% O2 treatment for 24hr) , *HT1080 , *786-0 , *FADU |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
