Application
HIF3 alpha antibody detects HIF3 alpha protein by western blot analysis. Non-transfected (-) and HIF3 alpha-transfected (+, inculding Myc / DDK-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF3 alpha antibody diluted at 1:5000.| Product Name | HIF3 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIF3 alpha (hypoxia inducible factor 3, alpha subunit)The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HIF3 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IPAS hypoxia inducible factor 3 alpha subunit HIF-3A bHLHe17 HIF3A MOP7 PASD7 |
|---|---|
| Target | HIF3 alpha |
| Molecular Weight(MW) | 72 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
HIF3 alpha antibody detects HIF3 alpha protein by western blot analysis. Non-transfected (-) and HIF3 alpha-transfected (+, inculding Myc / DDK-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF3 alpha antibody diluted at 1:5000.| Positive Control | HIF3A-transfected 293T cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
