Application
Antibody used in IHC on Human kidney.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | HIF3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HIF3A is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression.The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene.1) Kimura, K., (2006) Genome Res. 16 (1), 55-65. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HIF3A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| HIF3A antibody can be used for detection of HIF3A by ELISA at 1:312500. HIF3A antibody can be used for detection of HIF3A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | HIF3A |
|---|---|
| Alternative Names | HIF3A IPAS MOP7 PASD7 HIF-3A bHLHe17 |
| Molecular Weight(MW) | 72 kDa, 66 kDa, 72 kDa |
Application
Antibody used in IHC on Human kidney.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | HIF3A antibody can be used for detection of HIF3A by ELISA at 1:312500. HIF3A antibody can be used for detection of HIF3A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HIF3A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
