Application
Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human HIP/ST13 (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)Application
Cell lysates of 293T (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hip/ST13 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | HIP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT1F1] to HIPHIP(Hsc70-interacting protein), also known as ST13, is a co-chaperone to the major heat shock proteins, HSP70 and HSP90, and appears in early receptor complexes. Through mutual binding to both HSP70 and HSP90, Hip functions as an adaptor that can integrate HSP70 and HSP90 interactions. Also, Hip has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. |
| Immunogen | Recombinant human HIP (1-369aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT1F1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| Western Blot: Use at 1:1000 ~ 3000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HOP SNC6 Hsc70interacting protein Hsc70-interacting protein ST13 FAM10A1 Hsc70 interacting protein |
|---|---|
| Target | HIP |
| Light Chain Type | kappa |
Application
Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human HIP/ST13 (1:25) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Application
Cell lysates of 293T (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hip/ST13 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:1000 ~ 3000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
