Application
HIP1 Related antibody [N1N2], N-term detects HIP1R protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIP1R protein stained by HIP1 Related antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HIP1 Related antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000Application
HIP1 Related antibody [N1N2], N-term detects HIP1 Related protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HIP1 Related antibody [N1N2], N-term diluted at 1:500.| Product Name | HIP1R antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIP1R (huntingtin interacting protein 1 related) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HIP1R. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Rabbit, ChickenMouse (96%), Xenopus laevis (89%), Rabbit (95%), Chicken (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ14000 antibody FLJ27022 antibody HIP12 antibody HIP3 antibody ILWEQ antibody KIAA0655 antibody MGC47513 antibody HIP1R antibody huntingtin-interacting protein 12 antibody huntingtin-interacting protein 1-related protein antibody HIP1-related p |
|---|---|
| Molecular Weight(MW) | 119 kDa (note) (4) |
| Cellular Localization | Cytoplasm , perinuclear region , Endomembrane system , Cytoplasmic vesicle , clathrin-coated vesicle membrane |
Application
HIP1 Related antibody [N1N2], N-term detects HIP1R protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIP1R protein stained by HIP1 Related antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HIP1 Related antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:1000
Application
HIP1 Related antibody [N1N2], N-term detects HIP1 Related protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HIP1 Related antibody [N1N2], N-term diluted at 1:500.| Positive Control | MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
