Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Histone H1.0 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Histone H1.0 antibody at 1:500 dilution.Application
Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:500.Application
Histone H1 antibody detects Histone H1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H1.0 antibody detects Histone H1.0 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H1.0 antibody dilution: 1:500.Application
Histone H1 antibody detects Histone H1 protein by western blot analysis. HepG2 whole cell extracts and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody at a dilution of 1:10000 and developed with Trident femto Western HRP Substrate . The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Histone H1.0 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H1 (H1 histone family, member 0)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Histone H1.0. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H10 antibody H1FV antibody MGC5241 antibody H1F0 antibody histone H1.0 antibody H1.0 H1(0) H1-0 antibody histone H1(0) antibody histone H1' antibody H1 histone family member 0 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Histone H1.0 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Histone H1.0 antibody at 1:500 dilution.
Application
Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:500.
Application
Histone H1 antibody detects Histone H1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H1.0 antibody detects Histone H1.0 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H1.0 antibody dilution: 1:500.
Application
Histone H1 antibody detects Histone H1 protein by western blot analysis. HepG2 whole cell extracts and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody at a dilution of 1:10000 and developed with Trident femto Western HRP Substrate . The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 , HepG2 nuclear extract , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
