Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H1 (Acetyl-Lys25) Antibody. The picture on the right is treated with the synthesized peptide.Application
Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H1 (Acetyl-Lys25) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | Histone H1K25ac (acetyl Lys25) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H1K25ac (acetyl Lys25)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The protein encoded is a member of the histone H1 family. The related mouse gene is expressed only in oocytes. [provided by RefSeq, Jul 2008] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H1 around the acetylated site of Lys25. |
| Modification | acetyl Lys25 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | acetyl Histone H1K25ac K25 Histone H1K25ac Lys25 acetyl acetyl Lys25 Histone H1K25ac H1FOO Histone H1K25ac acetyl K25 Histone H1K25ac K25 acetyl Histone H1K25ac acetyl Lys25 acetyl K25 Histone H1K25ac Histone H1K25ac acetyl OSH1 |
|---|---|
| Target | Histone H1 |
| Molecular Weight(MW) | 21 kDa (note) |
| Tissue Specificity | Histone H1 (Acetyl-Lys25) Antibody detects endogenous levels of total Histone H1 protein only when acetylated at lysine25. |
Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H1 (Acetyl-Lys25) Antibody. The picture on the right is treated with the synthesized peptide.
Application
Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H1 (Acetyl-Lys25) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
