Application
ChIP was performed with HeLa nuclear extract and either 5 μg of H2Apan antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH promoter.Application
Dot blot analysis of peptide used for immunization and other peptides containing unmodified sequences of different histones using Histone H2A antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.Application
ELISA was performed using a serial dilution of Histone H2A antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,800.| Product Name | Histone H2A antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H2A |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAChIP |
| ChIP (1-2 µg/ChIP), ELISA (1:50), Dot blotting (1:20,000): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Histone H2A |
|---|
Application
ChIP was performed with HeLa nuclear extract and either 5 μg of H2Apan antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH promoter.
Application
Dot blot analysis of peptide used for immunization and other peptides containing unmodified sequences of different histones using Histone H2A antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
Application
ELISA was performed using a serial dilution of Histone H2A antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,800.| Application Notes | ChIP (1-2 µg/ChIP), ELISA (1:50), Dot blotting (1:20,000): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
