Application
Western Blot of A375, HEK293, HeLa and SK-MEL-2whole cell lysates, using RM214 at 0.5 ug/mL, showedendogenous Histone H2AX in A375, HEK293, HeLa andSK-MEL-2 cells.| Product Name | Histone H2A.X antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM214] to Histone H2A.XHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to the C-terminus of human Histone H2AX |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM214 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWBMultiplexing |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2AX H2AFX H2A.X H2A histone family member X H2A/X H2A.X antibody |
|---|---|
| Target | Histone H2A.X |
| Tissue Specificity | This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins. . |
Application
Western Blot of A375, HEK293, HeLa and SK-MEL-2whole cell lysates, using RM214 at 0.5 ug/mL, showedendogenous Histone H2AX in A375, HEK293, HeLa andSK-MEL-2 cells.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
