Application
Western Blot of acid extracts of HeLa cells treatedor non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylatedat serine 139 in HeLa cells.Application
Immunocytochemistry of HeLa cells using gammaH2AX Rabbit mAb RM224 (red). Actin filamentshave been labeled with fluorescein phalloidin (green).| Product Name | Histone H2A.XS139ph (phospho Ser139) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM224] to Histone H2A.XS139ph (phospho Ser139)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] |
| Immunogen | A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139) |
| Modification | p-Ser139 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM224 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWBMultiplexing |
| Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 2 ug/mL, Immunohistochemistry (IHC): 0.5 – 5 ug/mL, Immunocytochemistry (ICC): 0.5 – 6 ug/mL.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2A/X H2AX H2A.X H2AFX H2A histone family member X |
|---|---|
| Target | Histone H2A.X (phospho Ser139) |
| Tissue Specificity | This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. |
Application
Western Blot of acid extracts of HeLa cells treatedor non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylatedat serine 139 in HeLa cells.
Application
Immunocytochemistry of HeLa cells using gammaH2AX Rabbit mAb RM224 (red). Actin filamentshave been labeled with fluorescein phalloidin (green).| Application Notes | Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 2 ug/mL, Immunohistochemistry (IHC): 0.5 – 5 ug/mL, Immunocytochemistry (ICC): 0.5 – 6 ug/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
