Application
Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Histone H2A.Z antibody The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.Application
Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 μg of Histone H2A.Z antibody 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample(30 μg of whole cell lysate) A:MOLT4 B:Raji 12% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Histone H2A.Z antibody at 1:500 dilution.Application
Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Histone H2A.Z antibody diluted at 1:1000.| Product Name | Histone H2A.Z antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H2A.Z (H2A histone family, member Z)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.Z. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Dog, Pig, Chicken, Sheep, Rhesus Monkey, BovineRat (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2A.z antibody H2A/z antibody H2AZ antibody MGC117173 antibody H2AFZ antibody H2AZ histone antibody histone H2A.Z antibody H2A histone family member Z antibody |
|---|---|
| Molecular Weight(MW) | 14 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Histone H2A.Z antibody The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.
Application
Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 μg of Histone H2A.Z antibody 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample(30 μg of whole cell lysate) A:MOLT4 B:Raji 12% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Histone H2A.Z antibody at 1:500 dilution.
Application
Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Histone H2A.Z antibody diluted at 1:1000.| Positive Control | Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
