Application
RM216 specifically reacts to both Histone H2A and H4phosphorylated at Serine 1 (H2AS1p and H4S1p).Application
Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole. Using RM216 at 0.5 ug/mL, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.Application
Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H2A/H4 (Ser1) Rabbit mAb RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Product Name | Histone H2A/H4 (phospho Ser1) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM216] to Histone H2A/H4 (phospho Ser1) |
| Immunogen | An unmodified peptide corresponding to the N-terminus of Histone H3. |
| Modification | p-Ser1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM216 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWBMultiplexing |
| Western Blot: 0.5 ug/mL - 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 1 ug/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Histone H2A/H4 (phospho Ser1) |
|---|---|
| Tissue Specificity | This antibody reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones. |
Application
RM216 specifically reacts to both Histone H2A and H4phosphorylated at Serine 1 (H2AS1p and H4S1p).
Application
Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole. Using RM216 at 0.5 ug/mL, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.
Application
Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H2A/H4 (Ser1) Rabbit mAb RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Application Notes | Western Blot: 0.5 ug/mL - 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 1 ug/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
