Application
Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM238 at 0.5 ug/mL, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.Application
Immunocytochemistry of HeLa cells using Anti-Phospho-Histone H2B (Ser14) Rabbit mAb RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).| Product Name | Histone H2B (phospho Ser14) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM238] to Histone H2B (phospho Ser14) |
| Immunogen | A phospho-peptide corresponding to Phospho-Histone H2B (Ser14). |
| Modification | p-Ser14 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM238 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWBMultiplexing |
| Western Blot: 0.5 ug/mL - 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 1 ug/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Histone H2B (phospho Ser14) |
|---|---|
| Tissue Specificity | This antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones. |
Application
Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM238 at 0.5 ug/mL, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.
Application
Immunocytochemistry of HeLa cells using Anti-Phospho-Histone H2B (Ser14) Rabbit mAb RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).| Application Notes | Western Blot: 0.5 ug/mL - 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 1 ug/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
