Application
Western blot analysis of Hela cell lysate using 1 µg/ml Rabbit Anti-Histone 2B Polyclonal Antibody . The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.| Product Name | Histone H2B antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Histone H2BHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWBChIP |
| ICC/IF: Use at a concentration of 0.5 μg/ml. ChIP: Use at a concentration of 2-3 μg/ml. Every new batch of this antibody is tested at Abcam in ChIP.WB: Use at a concentration of 0.1 μg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).: |
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| Species Reactivity | HumanRatArabidopsisMammalian species |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2B.1B Histone H2B HIST1H2BD HIRIP2 MGC90432 dJ221C16.6 H2B/b H2BFB |
|---|---|
| Target | Histone H2B |
| Tissue Specificity | This antibody is specific for Histone 2B. |
| Cellular Localization | Nuclear |
Application
Western blot analysis of Hela cell lysate using 1 µg/ml Rabbit Anti-Histone 2B Polyclonal Antibody . The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.| Application Notes | ICC/IF: Use at a concentration of 0.5 μg/ml. ChIP: Use at a concentration of 2-3 μg/ml. Every new batch of this antibody is tested at Abcam in ChIP.WB: Use at a concentration of 0.1 μg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).: |
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| Form | Liquid |
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| Storage Instructions | Store at -20 °C or below long term. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Phosphate-buffered saline, pH 7.2 and 0.02% sodium azide |
