Application
Sandwich ELISA performed on the lysates of 293T cells overexpressing Histone H3.3 K36M mutant or Histone H3.3 wild type His-tag proteins, shows the specificity of RM193 to the K36M mutant. His-tag fusion proteins were captured by an Anti-His-Tag antibody.Application
Western Blot analysis of cell lysates prepared from 293T overexpressing the wild type or K36M mutant proteins of Histone H3.1 and H3.3.Application
293T cells overexpressing Histone H3 K36M mutant stained with anti-Histone H3 K36M clone RM193.| Product Name | Histone H3 (K36M Mutant Specific) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM193] to Histone H3 (K36M Mutant Specific) |
| Immunogen | A peptide corresponding to Histone H3 K36M mutant |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM193 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 2 ug/mL, Immunohistochemistry (IHC): 0.5 – 5 ug/mL, Immunocytochemistry (ICC): 0.5 – 5 ug/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Histone H3 (K36M Mutant Specific) |
|---|---|
| Tissue Specificity | This antibody reacts to the Histone H3 K36M mutant. No cross reactivity with wild type Histone H3. |
Application
Sandwich ELISA performed on the lysates of 293T cells overexpressing Histone H3.3 K36M mutant or Histone H3.3 wild type His-tag proteins, shows the specificity of RM193 to the K36M mutant. His-tag fusion proteins were captured by an Anti-His-Tag antibody.
Application
Western Blot analysis of cell lysates prepared from 293T overexpressing the wild type or K36M mutant proteins of Histone H3.1 and H3.3.
Application
293T cells overexpressing Histone H3 K36M mutant stained with anti-Histone H3 K36M clone RM193.| Application Notes | Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 2 ug/mL, Immunohistochemistry (IHC): 0.5 – 5 ug/mL, Immunocytochemistry (ICC): 0.5 – 5 ug/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
