Application
RM162 specifically reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p). Nocross reactivity with non-modified Lysine 9/ Serine 10,methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, or phosphorylation at Serine 9 ONLY in Histone H3.Application
Western Blot of acid extracts of HeLa cells (1) andrecombinant histone H3.3 (2), using RM162 at 0. 01 ug/mL, showed a band of histone H3 modified by both trimethylation at lysine 9 and phosphorylationat serine 10 (K9me3/S10p) in HeLa cells.| Product Name | Histone H3 (Tri-Methyl Lys9/Ser10) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM162] to Histone H3 (Tri-Methyl Lys9/Ser10) |
| Immunogen | A trimethyl-phospho-peptide corresponding to Trimethyl- Phospho-Histone H3 (Lys9/Ser10). |
| Modification | Tri-Methyl Lys9/Ser10 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM162 |
| Host Species | Rabbit |
| Tested Applications | ELISAWBMultiplexing |
| Western Blot: 0.01 ug/mL - 1 ug/mL, ELISA: 0.01 ug/mL - 0.5 ug/mL, Multiplex: 0.1 ug/mL – 1 ug/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Histone H3 (Tri-Methyl Lys9/Ser10) |
|---|---|
| Tissue Specificity | This antibody reacts to Histone H3 only when modified AT both trimethyl lysine 9 and phospho serine 10 (K9me3/S10p). |
Application
RM162 specifically reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p). Nocross reactivity with non-modified Lysine 9/ Serine 10,methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, or phosphorylation at Serine 9 ONLY in Histone H3.
Application
Western Blot of acid extracts of HeLa cells (1) andrecombinant histone H3.3 (2), using RM162 at 0. 01 ug/mL, showed a band of histone H3 modified by both trimethylation at lysine 9 and phosphorylationat serine 10 (K9me3/S10p) in HeLa cells.| Application Notes | Western Blot: 0.01 ug/mL - 1 ug/mL, ELISA: 0.01 ug/mL - 0.5 ug/mL, Multiplex: 0.1 ug/mL – 1 ug/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
