Application
Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [GT114] diluted at 1:500.Application
Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.Application
Histone H3 antibody detects Histone H3 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.Application
Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H3 antibody at 1:500 dilution.| Product Name | Histone H3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,IP,WB,ChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse,Drosophila,Monkey,Rat,Rice |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3/A antibody, H3FA antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST1H3A antibody, histone H3/j antibody, histone H3/i |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [GT114] diluted at 1:500.
Application
Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
Application
Histone H3 antibody detects Histone H3 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.
Application
Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H3 antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
