Application
RM186 specifically recognizes Histone H3 that is unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4.The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.Application
Western Blot of acid extracts of HeLa cells treated withsodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of histone H3 with unmodified Lys4.| Product Name | Histone H3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM186] to Histone H3 |
| Immunogen | An unmodified peptide corresponding to the N-terminus of Histone H3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM186 |
| Host Species | Rabbit |
| Tested Applications | ELISAWBMultiplexing |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | immunoglobulin heavy constant alpha 1 IgA1 IGHA1 Histone H3 antibody |
|---|---|
| Target | Histone H3 |
| Tissue Specificity | This antibody recognizes Histone H3 that is unmodified at Lys4 but does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3. |
Application
RM186 specifically recognizes Histone H3 that is unmodified at Lys4 and does not recognize acetylated, monomethylated, dimethylated, or trimethylated Lys4.The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
Application
Western Blot of acid extracts of HeLa cells treated withsodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 ug/mL, showed a band of histone H3 with unmodified Lys4.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
