Application
Western Blot of recombinant Histone H3.3 andHistone H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using RM188at 0.025 ug/mL, showed a band of recombinant H3.1 and H3.3 proteins, and total histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.Application
RM188 reacts to both Histone H3.1 and Histone H3.3.| Product Name | Histone H3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM188] to Histone H3Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to the C-terminus of human Histone H3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM188 |
| Host Species | Rabbit |
| Tested Applications | ELISAWBMultiplexing |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3.3A H3 histone family 3A H3F3A H3F3 Histone H3 antibody |
|---|---|
| Target | Histone H3 |
| Tissue Specificity | This antibody reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins. |
Application
Western Blot of recombinant Histone H3.3 andHistone H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using RM188at 0.025 ug/mL, showed a band of recombinant H3.1 and H3.3 proteins, and total histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.
Application
RM188 reacts to both Histone H3.1 and Histone H3.3.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
