Application
Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 Antibody .Application
Western blot analysis of extracts from 293 cells using Histone H3.1 Antibody .| Product Name | Histone H3.1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3.1Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Immunogen | Peptide sequence around aa.8~12 (R-K-S-T-G) derived from Human Histone H3.1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3/b H3FB HIST1H3D histone H3/j histone H3/i histone H3/d histone H3/a histone H3/h histone H3/k histone H3/l histone H3.1 histone 1 H3d histone H3/c histone H3/f H3 histone family member B histone H3/b histone cluster 1 H3d |
|---|---|
| Target | Histone H3.1 |
| Molecular Weight(MW) | 17 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 Antibody .
Application
Western blot analysis of extracts from 293 cells using Histone H3.1 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
