Application
Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys14) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | Histone H3K14ac (acetyl Lys14) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3 (Acetyl Lys14)Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys14. |
| Modification | acetyl Lys14 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3/j HIST1H3A H3/d histone H3.1 H3/k H3/c H3/i Histone H3 H3/f H31 H3/a H3/l H3/h Histone H3 (acetyl K14) Histone H3 (acetyl Lys14) Histone H3 acetyl K14 Histone H3 acetyl Lys14 Acetyl Histone H3 (K14) Acetyl Histone H3 (Lys14) |
|---|---|
| Target | Histone H3 |
| Molecular Weight(MW) | 15 kDa (note) |
| Tissue Specificity | Histone H3 (Acetyl-Lys14) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine14. |
Application
Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys14) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
