WB
Sample loading: Approximately 3 μ g of total protein gradient diluted from Arabidopsis and rice leaves. Antigen protein electrophoresis: 12% SDS-PAGE membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight blocking: 5% skim milk powder, room temperature incubation for 1 hour. Primary antibody: 1:5000 dilution, room temperature incubation for 1 hour.WB
Sample loading: Gradient diluted antigen protein, electrophoresis: 12% SDS-PAGE, membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight, blocking: 5% skim milk powder, incubation at room temperature for 1 hour, primary antibody: 1:5000 dilution, incubation at room temperature for 1 hour.| Product Name | Anti-Histone H3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G10980，P59169 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:1000-1:5000 IP:1:10-1:100 |
|
| Species Reactivity | ArabidopsisRice |
| Purification | Affinity purified |
| Gene Symbol | Histone H3 |
|---|---|
| Gene Synonyms | H3.3 HISTONE 3.3 HTR8 |
| Molecular Weight(MW) | 15.4 kDa |
WB
Sample loading: Approximately 3 μ g of total protein gradient diluted from Arabidopsis and rice leaves. Antigen protein electrophoresis: 12% SDS-PAGE membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight blocking: 5% skim milk powder, room temperature incubation for 1 hour. Primary antibody: 1:5000 dilution, room temperature incubation for 1 hour.
WB
Sample loading: Gradient diluted antigen protein, electrophoresis: 12% SDS-PAGE, membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight, blocking: 5% skim milk powder, incubation at room temperature for 1 hour, primary antibody: 1:5000 dilution, incubation at room temperature for 1 hour.| Application Notes | WB:1:1000-1:5000 IP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
