Application
Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4K20me1 antibody.Application
Dot-blot analysis of all sorts of methylation peptides using H4K20me1 antibody.Application
Immunofluorescence analysis of 293T cell using H4K20me1 antibody. Blue: DAPI for nuclear staining.| Product Name | Histone H3K20me1 (mono-methyl Lys20) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3K20me1 (mono-methyl Lys20)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide of human MonoMethyl-Histone H4-K20 |
| Modification | mono-methyl Lys20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-PIPWBChIP |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP assay: 1:20 - 1:100, CHIPseq: 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseRatOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 2 H4a H4 FO108 H4F2 HIST2H4A H4/n H4FN HIST2H4 |
|---|---|
| Target | Histone H3K20me1 (mono-methyl Lys20) |
Application
Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4K20me1 antibody.
Application
Dot-blot analysis of all sorts of methylation peptides using H4K20me1 antibody.
Application
Immunofluorescence analysis of 293T cell using H4K20me1 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP assay: 1:20 - 1:100, CHIPseq: 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
