Application
Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM230 at 0.2 ug/mLApplication
Immunocytochemical staining of HeLa cells, using Anti-Histone H2B Rabbit mAb RM230 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Product Name | Histone H2B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM230] to Histone H2BHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to the C-terminus of human Histone H2B. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM230 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWBMultiplexing |
| Western Blot: 0.1 ug/mL – 1 ug/mL, ICC: 0.5 μg/mL - 1 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.2 ug/mL – 1 ug/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 2 H2be GL105 H2B.1 H2BQ H2BGL105 HIST2H2BE H2BFQ H2B |
|---|---|
| Target | Histone H2B |
| Tissue Specificity | This antibody reacts to the Histone H2B protein, independent of post-translational modifications. No cross reactivity with other histone proteins. |
Application
Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM230 at 0.2 ug/mL
Application
Immunocytochemical staining of HeLa cells, using Anti-Histone H2B Rabbit mAb RM230 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Application Notes | Western Blot: 0.1 ug/mL – 1 ug/mL, ICC: 0.5 μg/mL - 1 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.2 ug/mL – 1 ug/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
