Application
Western blot analysis of lysed extracts from HeLa and 293 cells using H2B Antibody.| Product Name | H2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105 |
| Product description | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.1) Borchert GM, et al. (2010) PLoS One. 5 (7): e11641. Pennini ME, et al. (2010) PLoS Pathog. 6 (7): e1000995. Lu C, et al. (2010) Sci China Life Sci. 53 (6): 663-8. |
| Immunogen | H2B antibody was raised against a peptide sequence around aa.41~45 (Y-V-Y-K-V) derived from Human H2B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIST2H2BE |
|---|---|
| Alternative Names | H2B H2BQ GL105 H2B.1 H2BFQ H2BGL105 |
| Molecular Weight(MW) | 14 kDa |
| Tissue Specificity | This antibody detects endogenous level of total H2B protein. |
| Entrez Gene | 8349 |
|---|---|
| Protein Accession | NP_003519.1 |
Application
Western blot analysis of lysed extracts from HeLa and 293 cells using H2B Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
